Introduction. Previous studies for color preference are mostly limited to choices of one or two color. We used a novel stimulus, a combination of two adjacent squares with a narrow rectangle (border) in between to assess the effect of a third color in the preference for a color combination. We used this method to evaluate the distance of the border color and two peripheral colors in the DKL color space as a predictor for preference. Method. Stimuli consist of all 504 combinations of 9 selected points in the DKL space. On a rectangular representation of DKL with gray in the middle, 9 points were selected including 4 corners, the center and 4 mid-sides. Each possible combination compared with the rest in a forced two choice task in 3024. 50 subjects (25 female and 25 male) in the range of 20-40 years old participated in the experiment. Results. In general, the greater the sum of distances between colors correlated with more preference in the combinations (T= 3.585, p-value=0.00). In addition, if the border line settles in longer distance from two other colors, it was preferred more (R= -0.726 , p-value= 0.00). Conclusion. Distant border color is more preferred in a triple color combination.